The Zika virus is transmitted by the Aedes mosquito - found in tropical climates, such as Central and South America and Africa. The Zika outbreak is currently mainly in the Americas, with countries such as Brazil being most affected.
what is Zika virus ?
Zika virus disease is mainly spread by mosquitoes. For most people it is a very mild infection and isn't harmful. However, it may be more serious for pregnant women and their partners (Zika found in the semen), as there's evidence it causes birth defects – in particular, abnormally small heads (microcephaly). Zika outbreaks have been reported in the Pacific region, and the virus has now spread to South and Central America and the Caribbean.
It is recommended that pregnant women postpone non-essential travel to areas with active Zika virus transmission. If you travel to an affected area, you can reduce your risk of catching the virus by using insect repellent and wearing loose clothing that covers your arms and legs.
Symptoms of Zika virus infection
Most people don't have any symptoms. If symptoms do occur, they are usually mild and last around two to seven days. Commonly reported symptoms include: rash, itching all over the body, fever, headache, joint and muscle pain, conjunctivitis (red eyes), pain behind the eyes
HOW ZIKA VIRUS SPREADS:
The mosquitoes that carry Zika are aggressive daytime biters, but they can also bite at night. A mosquito becomes infected when it bites a person already infected with Zika. That mosquito can then spread the virus by biting more people. Zika virus can also spread:
#From a pregnant woman to her fetus during pregnancy or around the time of birth
#Through blood transfusion
#During zex with a man infected with Zika.
Zika has been found in the semen of men who have been exposed to the Zika Virus. There have been reported cases of zexual transmission of the virus from men to women, and cases of the virus spreading to people who have not been to high risk countries. It is not known how long the virus may be present in semen, or how long after exposure the virus can be transmitted in semen. If you have been exposed to the virus, and your partner is pregnant, you should get tested and use condoms for 28 days from return if you do not have symptoms, and six months if you do have symptoms.
Advice for pregnant women
There is enough evidence to show that Zika virus infection is a cause of birth defects, including microcephaly (this means the baby will have an abnormally small head and can be associated with abnormal brain development). This is also known as congenital Zika syndrome.
It is therefore recommended that pregnant women postpone non-essential travel to areas with active Zika transmission until after pregnancy.
Advice for women trying to get pregnant
#If you are trying to get pregnant, You should take extra care to avoid being bitten by mosquitoes.
#It is recommended that you avoid becoming pregnant while travelling to an area with active Zika virus transmission, and for eight weeks after you return home.
#If you have experienced Zika symptoms within two weeks of returning home, it is recommended that you wait at least eight weeks after full recovery before you try to get pregnant.
#If your partner has travelled to an area with active Zika virus transmission, you should use effective contraception to prevent pregnancy and condoms during zex to reduce the risk of sexual transmission. These measures should be taken during travel and:
#for eight weeks after his return home, even if he has no Zika symptoms
#for six months following the start of his symptoms, if he does experience Zika symptoms or a Zika virus infection has been confirmed by a doctor
One step Zika Virus IgG/IgM Combo Test is a rapid immunochromatographic assay for the simultaneous detection of IgG and IgM antibodies to Zika virus in human whole blood, serum or plasma. The assay is used as screening test for zika viral infection.
The Zika IgG/IgM Rapid Test is a qualitative membrane strip based immunoassay for the detection of Zika virus antibodies (IgG and IgM) in Whole Blood /Serum / Plasma. The test device consists of:
1) a burgundy colored conjugate pad containing Zika recombinant envelope antigens conjugated with Colloid gold (Zika conjugates), Zika recombinant envelope antigens conjugated with Colloid gold (Zika conjugates),
2) a nitrocellulose membrane strip containing two test bands (T1 and T2 bands) and a control band (C band).
The T1 band is pre-coated with the antibody for the detection of IgM anti- Zika, T2 band is coated with antibody for the detection of IgG anti- Zika. When an adequate volume of test specimen is dispensed into the sample well of the test cassette, the specimen migrates by capillary action across the cassette. IgG anti- Zika, if present in the specimen, will bind to the Zika conjugates. The immunocomplex is then captured by the reagent pre-coated on the T2 band, forming a burgundy colored T2 band, indicating a Zika IgG positive test result and suggesting a recent or repeat infection. IgM anti- Zika if present in the specimen will bind to the Zika conjugates. The immunocomplex is then captured by the reagent coated on the T1 band, forming a burgundy colored T1 band, indicating a Zika IgM positive test result and suggesting a fresh infection. Absence of any T bands (T1 and T2) suggests a negative result. To serve as a procedural control, a colored line will always appear at the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred.
• The test can be used to test Whole Blood /Serum/Plasma specimens.
• To collect whole blood, serum or plasma specimens following regular clinical laboratory procedures.
1. Remove the test device from the sealed pouch and use it as soon as possible.
2. Place the test device on a clean and level surface.
3. Hold the dropper vertically and transfer 1 drop of specimen (approximately 10?l) to the specimen well(S) of the test device, then add 2 drops of buffer (approximately 70?l) and start the timer. See illustration below.
4. Wait for the colored line(s) to appear. Read results at 15 minutes. Do not interpret the result after 20 minutes.