Anti sperm antibody test + Anti ovary antibody test , infertility test
Anti sperm antibody [IgM+IgA+IgG] + Anti ovary antibody [IgM+IgG]
Between 10 to 15% of all couples in America have trouble conceiving, which can stem from a wide range of causes. One of the those you may not have heard of is antisperm antibodies: immune proteins that fight against the sperm cells.
Antibodies are cells in the immune system that attack invading cells like bacteria and viruses. However, antibodies can sometimes mistakenly attack cells that are supposed to be in your body too, causing problems. Such is the case with anti-sperm antibodies and anti-ovarian antibodies, which can cause infertility
How does antisperm antibody form?
A man’s blood and sperm do not come in contact with each other because of a barrier system (Testis-blood barrier). In testis, specialized cells known as Sertoli cells form this barrier. Any disturbance to this system physical, chemical, infection, inflammation and trauma could break the barrier and sensitize the immune system to sperm. Sometimes infection or inflammation in other parts of male genital system could produce the antibodies.
Antisperm antibody and male infertility
A man’s sperm is usually separated from the immune system by a physical barrier in the testicles. However, surgery like a vasectomy, a physical injury or an infection that brings the sperm in contact with the blood can expose the sperm to the immune system, causing it to develop antisperm antibodies.
Once the immune system develops these antibodies, it will mistakenly recognize the man’s own sperm as a foreign invader and try to slow down or immobilize it. This can result in poor sperm motility, which can cause infertility in men.
Antisperm antibody and female infertility
In order for a woman to get pregnant, a sperm cell from the father has to combine with and fertilize an egg cell from the mother. However, in some cases, the woman’s immune system recognizes the sperm cells as foreign bodies and begins to attack them.
The cause of antisperm antibodies in women’s body is still unknown to science, and sperm cells don’t usually trigger an immune response in the women. When they do, however, it can cause big problems for women trying to conceive.
ANTI OVARIAN ANTIBODY
Anti ovarian antibodies are typically found in women with premature ovarian failure. Large numbers of women with unexplained infertility also have anti ovarian antibodies in their blood. Anti ovarian antibodies in them are associated with poor implantation, higher rates of IVF treatment failure and poor response of hormone therapy.
Anti-Ovarian Antibodies (AOA) test measures the amount of AOA in the blood of a patient. AOA is most often found in cases of unexplained infertility. Elevated levels of AOA may impair the body’s ability to respond to attempts at ovulation induction
1. Take out the kit and put it at room temperature for 20~30 min;
2. Dispense 1 drop of Wash Buffer to the test window, waiting for the liquid to wet the membrane well;
3. Add 150?l of serum (4 drops if with a sample pipet) into the test window, waiting for the liquid to be absorbed completely;
4. Add 3 drops of Colloidal Gold Conjugate into the test window, waiting for the liquid to be absorbed enough;
5. Add 3 drops of Wash Buffer into the test window, and interpret the result within 3 minutes immediately after the liquid is absorbed adequately
Note1: This is SERUM / plasma TEST only,
You need to be able to separate SERUM / plasma from blood, to perform this test.